The Harris lab recently published an article in Nature Scientific Data entitled “A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development”. The work reveals 3D reconstruction from serial section electron microscopy from volumes of CA1 of the rat hippocampus. These data set guidelines for object identification and preparing dense reconstructions are presented provide standards for identifying ultrastructural objects in 3DEM, realistic reconstructions for modeling biophysical properties of synaptic transmission, and a test bed for enhancing reconstruction tools.
Harris KM, Spacek J, Bell ME, Parker PH, Lindsey LF, Baden AD, Vogelstein JT, Burns R. Sci Data. 2015 Sep 1;2:150046. doi: 10.1038/sdata.2015.46.